Immunocytochemistry (ICC) Protocol for Intestinal Organoids. Protocol 1. Passage organoids into 50 μL domes made of 50% ...
Required: - Liberase (Research Grade from Sigma-Aldrich Cat#05401119001) - Ammonium Chloride Solution (STEMCELL Cat#0780...
小鼠肠道类器官可使用 IntestiCult™类器官生长培养基(小鼠)(产品号#06005)进行构建并维持长期培养。下文中列出了确保成功分离小鼠肠道隐窝和培养小鼠肠道类器官的关键步骤。常规提示:在整个过程中,在操作肠段或者隐窝之前,应预先将...
配方基于发表的两篇文献,点击可下载文献原文•Sato et al. Long-termexpansion of epithelial organoids from human colon, adenoma, adenocarcinoma,a...
配方基于发表的两篇文献,点击可下载文献原文•Sato et al. Single Lgr5 stem cells buildcrypt-villus structures in vitro without a mesenchymal nic...
本文档含有:分化肠类器官,建立单层肠上皮培养体系,冻存和复苏肠类器官的实验流程。‍中文PDF下载‍ 英文PDF下载
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Mouse Intestinal Organoid Culture with IntestiCult™ Organoid Growth Medium
本视频展示了使用STEMCELL#06005培养小鼠肠类器官